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High-throughput screening, the subject of this chapter, has as its first objective the identification of a few ‘validated hits’ (defined in Chapter 9) within large compound libraries. The decision as to whether a particular hit is worth pursuing as a chemical lead in a drug discovery project depends on several factors, important ones being
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High-throughput screening, the subject of this chapter, has as its first objective the identification of a few ‘validated hits’ (defined in Chapter 9) within large compound libraries. The decision as to whether a particular hit is worth pursuing as a chemical lead in a drug discovery project depends on several factors, important ones being
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2020-8-6 Enzymes are common targets in high-throughput screening and related campaigns. An analysis of s published between 1990 and 2018 showed that kinases were the most common enzymes investigated, fluorescence-based assays were the most common readout method, and cancer and bacterial infections were the most common therapeutic areas. High-throughput screening and fragment-screening
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In these respective subject areas, Combinatorial High Throughput Screening is intended to function as the most comprehensive and up-to-date medium availe. The journal should be of value to individuals engaged in the process of drug discovery and development, in the settings of industry, academia, or government.
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High-Throughput Screening (HTS) is an approach to drug discovery that has gained widespread popularity over the last two decades and has become a standard method for drug discovery in the
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2013-11-14 aspects of high throughput screening data interpretation. 2. Role of statistics in HT screening design 2.1. HT screening process A typical HT screening campaign can be divided into five major steps regardless of the as‐ say type and the assay read-out (Fig. 1). Once target or pathway is identified, assay develop‐
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Miniaturisation of assays for high throughput screening As a result of the increasing number of chemical compounds and molecular targets for HTS, there has been a trend toward the miniaturisation of assays to enhance assay throughput and screen times. 4 The first major development was the 384-well plate, with a standard volume of around 50 μl, most assays can be easily adapted to this format.
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Summary of 24 pages for the course High Throughput Screening at UvA Complete summary ( ) Studies, courses, subjects, and textbooks for your search: Press Enter to view all search results ( )
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Profiling Anticancer and Antioxidant Activities of Phenolic Compounds Present in Black Walnuts ( Juglans nigra) Using a High-Throughput Screening Approach Molecules. 2020 Oct 2;25(19):E4516. doi: 10.3390/molecules25194516.
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High-Throughput Sequencing of Bacterial Communities. Partial 16S rRNA gene (hypervariable V3-V4) libraries were amplified using universal bacterial primers 341F (forward) and 805R (reverse) (Klindworth et al., 2013). Each forward and reverse primer contained Illumina overhang adapters (Illumina Inc.,
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2016-11-4 high throughput screening methods including biochemical and molecular markers are being investigated to improve the selection process and eliminate genotypes with poor quality potential before the expensive mini manufacture stage. A call is made for the establishment of a Global Tea Research Project to address common problems.
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Dalu Mancama is a principal Council for Scientific and Industrial Research (CSIR) researcher and research group leader for the bioassays and high-throughput screening research team. His qualifications include an BSc in applied biochemistry from Brunel University and specialisation in molecular cell biology and genetics through an MSc and PhD from the University of London Colleges of Imperial
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2015-2-20 The tetrazolium-based MTT assay has long been regarded as the gold standard of cytotoxicity assays as it is highly sensitive and has been miniaturised for use as a high-throughput screening assay. However, various reports refer to interference by different test compounds, including the glycolysis inhibitor 3-bromopyruvate, with the conversion of the dye to coloured formazan crystals.
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Tantoh, a cell biologist with extensive experience in screening technology development, leads the high-content screening platform at the CSIR. He has a Master’s degree in molecular biochemistry from Stellenbosch University.
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High-Throughput Sequencing of Bacterial Communities. Partial 16S rRNA gene (hypervariable V3-V4) libraries were amplified using universal bacterial primers 341F (forward) and 805R (reverse) (Klindworth et al., 2013). Each forward and reverse primer contained Illumina overhang adapters (Illumina Inc.,
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2016-11-4 high throughput screening methods including biochemical and molecular markers are being investigated to improve the selection process and eliminate genotypes with poor quality potential before the expensive mini manufacture stage. A call is made for the establishment of a Global Tea Research Project to address common problems.
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Dalu Mancama is a principal Council for Scientific and Industrial Research (CSIR) researcher and research group leader for the bioassays and high-throughput screening research team. His qualifications include an BSc in applied biochemistry from Brunel University and specialisation in molecular cell biology and genetics through an MSc and PhD from the University of London Colleges of Imperial
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Background: The tetrazolium-based MTT assay has long been regarded as the gold standard of cytotoxicity assays as it is highly sensitive and has been miniaturised for use as a high-throughput screening assay. However, various reports refer to interference by different test compounds, including the glycolysis inhibitor 3-bromopyruvate, with the conversion of the dye to coloured formazan crystals.
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2015-2-20 The tetrazolium-based MTT assay has long been regarded as the gold standard of cytotoxicity assays as it is highly sensitive and has been miniaturised for use as a high-throughput screening assay. However, various reports refer to interference by different test compounds, including the glycolysis inhibitor 3-bromopyruvate, with the conversion of the dye to coloured formazan crystals.
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Further enhancement of quality may then be achieved by high throughput cultivar screening where those with a higher natural catechin to caffeine content will be favourable. Six different PPO inactivation methods (steaming, blanching, fluid bed drying, panfrying, grilling over direct heat and grilling over indirect heat) were explored.
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2016-11-4 high throughput screening methods including biochemical and molecular markers are being investigated to improve the selection process and eliminate genotypes with poor quality potential before the expensive mini manufacture stage. A call is made for the establishment of a Global Tea Research Project to address common problems.
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2020-10-7 BACKGROUND: Despite increased prevention efforts, HIV remains the leading cause of death among adolescent girls and young women in South Africa. Although research indicates important determinants of HIV acquisition at the individual and interpersonal levels, structural-level stigma and discrimination continue to be critical barriers to reaching and retaining this key population for HIV
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Naicker’s research interest include the development of point-of-care diagnostic and vaccine-matching tests for livestock diseases, as well as the development of high-throughput screening and automatable tools for accelerating proteomics research.
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Further enhancement of quality may then be achieved by high throughput cultivar screening where those with a higher natural catechin to caffeine content will be favourable. Six different PPO inactivation methods (steaming, blanching, fluid bed drying, panfrying, grilling over direct heat and grilling over indirect heat) were explored.
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2020-4-24 RTI International has ed in South Africa for 30+ years, providing technical assistance, institutional strengthening, program support, and research. We design and implement programs across a wide range of sectors to help low- and middle- income countries and communities address complex problems and improve the lives of their citizens.
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